Wednesday, April 23rd, 2025

Agronomy 3140, 3:00 – 4:00 pm

“Development of a Single Kernel NIR Prediction Equation for the Selection of Waxy Maize Kernels”

Shelly Kinney,

Seminar Description: The waxy gene of maize is a high value breeding target, but it is time consuming to separate waxy and wild-type kernels. A common method involves staining the endosperm with iodine. Near-infrared reflectance (NIR) spectroscopy has been used in several species including maize with success. A custom-built single kernel NIR spectroscopy machine was used to scan 2880 individual kernels from 60 samples with a diversity of pedigrees, with both waxy, wild type, and heterozygous kernels represented. Chemical analysis was performed to classify the kernels with the waxy or wild type phenotypes. Linear discriminant analysis was conducted to develop a prediction equation for single kernel NIR spectroscopy. The discriminant results showed that there was an 88% accuracy in predicting waxy kernels as waxy, and a 96% accuracy in predicting wild type kernels as wild type. An ROC curve was determined to allow threshold adjustment to meet desired true positive or false negative rates. Thus, the prediction equation can be used in breeding programs to select for waxy kernels in an efficient and effective manner using a single kernel NIR machine. This approach will benefit breeders of waxy corn by providing a rapid, automated non-destructive method for identification of waxy kernels in segregating breeding populations.

Shelly Kinney grew up in East Central Minnesota, and started her academic career by graduating from Pine Technical and Community College with an associate’s degree in 2018. Afterwards, she majored in Biology for her bachelor’s degree, graduating from the University of Wisconsin-Superior in 2021. She was then accepted into Iowa State University’s Interdepartmental Genetics and Genomics program for a master’s degree, which she completed in 2024. She is now continuing at ISU with a PhD in Plant Breeding.