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Raymond F. Baker Center for Plant Breeding

Thomas Lübberstedt'S Projects

Thomas Lübberstedt's Projects

  • Corn kernels haploid

    The objectives of our program are to (i) optimize DH technology in maize regarding haploid induction, haploid identification, and spontaneous haploid genome doubling, (ii) employ DH technology, e.g., to establish exotic introgression DH lines in collaboration with the USDA gene bank in Ames, and (iii) transfer knowledge to other species. PI Lubberstedt, T. Funding: K.J. Frey Chair in Agronomy, R.F. Baker Center for Plant Breeding, Plant Sciences Institute, USDA OREI, USDA SCRI, NSF P3, FFAR & breeding companies.

  • Microfluidic device for cell sorting

    The ability to double haploid genomes spontaneously would make DH technology more efficient, while avoiding use of toxic colchicine. Spontaneous haploid genome doubling (SHGD) occurs at an extremely low frequency in maize germplasm. The first goal (Goal 1) is isolation of the gene underlying a major QTL for SHGD in maize and to evaluate it in various crop species. A quantum leap in the breeding timeline proposed here is the concept of an in vitro nursery (IVN). Selected genotypes can be maintained within minimal lab-space using cell cultures.Our 2nd goal (Goal 2) is to initiate a systematic approach towards development of in vitro nurseries in any species of interest.